2-Needle Array Electrode User's Manual
The 2-Needle Array™ Electrode is a needle style electrode specifically designed for in-vivo gene delivery in muscles. The electrode consists of a reusable 2-Needle Array Handle and disposable two-needle array tips. The needle array tips are supplied in packs of six.
Quick Start:
To secure the needle array tip to the Needle Array Handle:
- Position the handle over a needle array tip and push to secure onto the handle.
- Attach the handle to a BTX electroporator via the high voltage banana cables.
- Remove the needle safety shield, place into the tissue, and deliver the pulse.
- Discard the needle array tip and prepare for the next experiment.
The 2-Needle Array™ Electrode comes in 5mm and 10mm electrode spacing and is made from medical grade stainless steel.
| Order No. | Model | Description |
| 45-0167 | 2-Needle Array, 10 mm kit | |
| 45-0205 | 530 | 2-Needle Array Handle, 10 mm gap |
| 45-0120 | 531 | 2-Needle Array Tip, 10 mm gap, pkg.of 6 |
| 45-0168 | 2-Needle Array, 5 mm kit | |
| 45-0206 | 532 | 2-Needle Array Handle, 5 mm gap |
| 45-0121 | 533 | 2-Needle Array Tip, 5 mm gap, pkg. of 6 |
Harvard Bioscience, Inc. holds the following patents:
U.S. Patent #5, 273, 525
U.S. Patent #5, 702, 359
For Research Purposes Only.
WARNING! HIGH VOLTAGE
Getting Started
Make sure the BTX electroporator is switched off before continuing.
- Grasping the 2-Needle Array Handle, position the handle over a 2-Needle Array Tip and push to secure the 2-Needle Array to the handle.
- Attach the handle to a BTX electroporator via the high voltage banana cables.
- Following instructions for the electroporator set the appropriate parameters on the generator.
- Prepare tissue and sample for electroporation. Apply sample to tissue just before electroporation.
- Remove the safety shield protecting the needles and immerse needles in >95% ethanol for 15 seconds to sterilize the needles prior to use.
- Insert the needles into the tissue and deliver the electroporation pulse.
- Discard the 2-Needle Array Tip in a typical biohazard sharps container. Refer to your country's waste management system for proper disposal practices.
- Clean the 2-Needle Array Handle with a soft cloth or tissue. If necessary, moisten the cloth or tissues with a dilute detergent solution.
Note: The 2-Needle Array Tips are designed for single use. If re-using these electrodes, sterilize them with ethanol or ethylene oxide (ETO). Do Not Autoclave. Maximum usage is three sets of pulses.
Specifications
Standard Capabilities:
| Voltage Range | 0 to 500 VDC |
| Pulse Length/Time Constants Range | 1 µsec to 99 msec |
| Pulse Number Range | 1 to 99 |
| Operating Temperature | 5° to 40°C |
| Intended Use | Indoor Use Only |
| Relative Humidity | 20 to 80% |
| Maximum Altitude | 2,000 m (6562 ft) |
| Pollution Degree | II |
| Insulation Category | CAT I |
Physical Characteristics:
| Model | 530/531 | 532/533 |
| Needle Spacing | 10 mm | 5 mm |
| Needle Length | 20 mm | 20 mm |
| Needle Material | Stainless Steel | Stainless Steel |
| Handle Length | 8 cm | 8 cm |
Compatibility:
ECM®630, 830 and 2001 models
Replacement Part Reference:
| Order No. | Model | Description |
| 45-0167 | 530KIT | 20-Needle Array Kit, 10 mm |
| 45-0205 | 530 | 2-Needle Array Handle, 10 mm gap |
| 45-0120 | 531 | 2-Needle Array Tip, 10 mm gap, pkg. of 6 |
| 45-0168 | 532KIT | 2-Needle Array Kit, 5 mm |
| 45-0206 | 532 | 2-Needle Array Handle, 5 mm gap |
| 45-0121 | 533 | 2-Needle Array Tip, 5 mm gap, pkg. of 6 |
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