Introduction

The Tweezertrodes are reusable, tweezer style electrodes for in vivo, and in utero drug or gene delivery. Tweezertrodes consist of a standard 12cm tweezer that has been modified with stainless steel or platinum circular electrodes at the tip. The gap between the electrode disks may be adjusted from under 1mm to over 2cm. Tweezertrodes are available in two sizes for stainless steel 10mm and 7mm diameters. Our Platinum Tweezertrodes are available in 7mm, 5mm, 3mm and 1mm diameters. Tweezertrodes may be cleaned with a mild detergent and sterilized with athanol or ethylene oxide. These electrodes are connected to an electroporator with the Model 524 Connection Cable, and are compatible with the BTX ECM 630, 830, Gemini, 2001+ and 2001 Lite. 

Operation: Getting Started

Make sure the BTX electroporator is switched off before continuing.

1. Attach the Model 524 Tweezertrodes connection cable to the electrode base of the Tweezertrodes then plug the banana calbe into the voltage output of the BTX electroporator. If using the Enhancer 3000 attach the cables to the Enhancer and then attach the cables from the Enhancer to the Generator. Refer to the Enhancer 3000 for instructions for use.

2. Following the instructions for the BTX electroporator to set the appropriate parameters on the generator.

3. Prepare tissue and sample for electroporation. Grasp the tissue between the electrodes and measure the inter-electrode distance. Adjust generator settings if necessary. Inject the sample into the tissue. 

4. Deliver the electroporation pulse(s) to the sample.

5. Remove the electrodes carefully and prepare for the next experiment. 

Cleaning

The Tweezertrodes can be cleaned and disinfected using a solution of 70% isopropanol. 

Specifications:

* Depending on buffer composition and generator capability.

Warranty

BTX-Harvard Apparatus warranties the BTX Tweezertrodes for a period of 90 days from date of purchase. For additional terms please see Return Policy here.