"Multiple Errors" means precisely that; that more than one issue is happening at a time with the instrument and all errors cannot be displayed at once. Unfortunately this does not give information about what the identity of multiple errors are; just that there are more than one error occurring. Getting some additional information from the customer about their instrument settings and their sample prep is crucial to figuring out whether this is an instrument issue or an applications issue and how best to resolve it.

1. Please submit to Technical Support your electroporation protocol (waveform, pulse length for square wave/or resistance + capacitance for exponential decay, number of pulses, etc.) Do you use the instrument in HT plate handler mode to automatically cycle through the plate, or one column at a time?

2. What are the HT-200 plate handler settings? What column does the plate handler start on, and what is the pulse count?

3. What electroporation buffer and volume was used in the electroporation plate? What is the transfectant (for example DNA, RNA) dissolved in, and what volume?

4. What is the frequency of this issue? Does the instrument give this error on every attempt/every column of the plate or just some of the columns?

5. What was the sample resistance value measured during the pre-pulse? (You can push the ohm button to check this, and additionally is measured and recorded in the instrument log with the electroporation protocol)

6. If you run the same settings on the instrument and plate handler with no plate inserted into the HT-200, do errors stay the same?-this test helps us to determine whether the sample is contributing to the issue.